Search found 5 matches

by DetnPx
Mon Jun 11, 2018 12:37 am
Forum: Forum
Topic: ASC vs. O.C. $1K aldehyde bath + molecular brain scan
Replies: 9
Views: 448

Re: ASC vs. O.C. $1K aldehyde bath

Although I'm not a supporter of the ASC and/or uploading approaches, I can imagine that "scanning tissue on the molecular scale" in ~50 years would simply mean an injection of nanoscale robots which work their way through tissue and independantly transmit what structure they come across, t...
by DetnPx
Sun Apr 08, 2018 1:51 pm
Forum: Forum
Topic: Higher concentration of cryoprotectant
Replies: 6
Views: 693

Re: Higher concentration of cryoprotectant

I concur, this could be another serious concern that needs some added research. Thank you for raising the issue.
by DetnPx
Sun Apr 08, 2018 12:50 pm
Forum: Forum
Topic: Higher concentration of cryoprotectant
Replies: 6
Views: 693

Re: Higher concentration of cryoprotectant

50/50 Ethylene glycol / DMSO: 5% increments, cooled to -80°C. Concentrations of 55% and below were frozen, while 100% was partly frozen. Ranges from 60% to 95% remained clear and syrupy, not very viscous at all. -80°C is just not cold enough. Did you, coincidentially, also leave these vials at -80C...
by DetnPx
Mon Dec 18, 2017 1:22 am
Forum: Forum
Topic: Intermediate Temperature Storage
Replies: 7
Views: 943

Re: Intermediate Temperature Storage

Interesting. Perhaps a specially (industry)designed vacuum-insulated sealed polymer/fiber container around each specimen? This might provide the most insulation vs. space benefit compared to traditional insulation such as XPS. Where does the LN2(l) go, is it at the bottom of the actual storage area,...
by DetnPx
Sun Dec 17, 2017 3:34 pm
Forum: Forum
Topic: Intermediate Temperature Storage
Replies: 7
Views: 943

Re: Intermediate Temperature Storage

Hi Jordan,

Does your new ITS unit maintain a homogenous temperature throughout the vertical space of the dewar, and if so, how does it do this? And what are the critical electronic parts in the ITS that could cause a failure and temperature rise, besides the thermostat?

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