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I've decided it's time to increase the concentration of cryoprotectant that we use. We've been using 65%, but we're going to move closer to 75 or 80%. Higher concentrations have the very important advantage completely preventing all ice formation, regardless of temperature or cooling rate. This will also allow safe storage at intermediate temperatures of about -125 without any risk of ice nucleation. This approach is known as equilibrium vitrification, and it only works for us because we chemically fix the tissue first, so toxicity at the higher concentration is not an issue.